1SJH

HLA-DR1 complexed with a 13 residue HIV capsid peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-03-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	172.464, 172.464, 121.461
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.250
R-factor	0.22
Rwork	0.220
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pyw
RMSD bond length	0.006
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.250	2.250
Rmerge	0.127	0.492
Number of reflections	64060
<I/σ(I)>	9.6	1.9
Completeness [%]	99.8	99.8
Redundancy	4.5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	277	PEG 4000, Ethylene glycol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K