1SJC
x-ray structure of o-succinylbenzoate synthase complexed with N-succinyl methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-07 |
Wavelength(s) | 0.6525 |
Spacegroup name | H 3 2 |
Unit cell lengths | 215.700, 215.700, 259.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.211 |
Rwork | 0.208 |
R-free | 0.27400 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 2.260 |
Data reduction software | HKL-2000 |
Data scaling software | d*TREK |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 154522 | |
<I/σ(I)> | 37.6 | 3 |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 9.9 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | batch | 8 | 298 | PEG 8000, HEPES, MgCl2, n-succinylmethionine, pH 8.0, batch, temperature 298K |