1SJB
X-ray structure of o-succinylbenzoate synthase complexed with o-succinylbenzoic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-29 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 216.000, 216.000, 261.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.209 |
| Rwork | 0.204 |
| R-free | 0.27200 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.170 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 116834 | |
| <I/σ(I)> | 19 | 1.8 |
| Completeness [%] | 98.7 | 95.7 |
| Redundancy | 5.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 8 | 298 | PEG 8000, HEPES, MgCl2, o-succinylbenzoic acid, pH 8.0, batch, temperature 298K |
