1SJA
X-ray structure of o-Succinylbenzoate Synthase complexed with N-acetylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-28 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 215.300, 215.300, 259.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.211 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 107728 | |
| <I/σ(I)> | 28.1 | 1.8 |
| Completeness [%] | 99.2 | 96.4 |
| Redundancy | 7.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 8 | 298 | PEG 8000, HEPES, MgCl2, N-acetylmethionine, pH 8.0, batch, temperature 298K |
