1SJ9
Crystal structure of the uridine phosphorylase from Salmonella typhimurium at 2.5A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.803 |
| Spacegroup name | P 61 |
| Unit cell lengths | 91.370, 91.370, 266.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.340 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.063 | 0.221 |
| Number of reflections | 43054 | |
| <I/σ(I)> | 14.03 | 6.18 |
| Completeness [%] | 99.4 | 97 |
| Redundancy | 3.72 | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | sodium-acetate trihydrate, PEG 10000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
