1SJ5

Crystal structure of a duf151 family protein (tm0160) from thermotoga maritima at 2.8 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	90
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	44.001, 52.136, 73.330
Unit cell angles	90.00, 97.64, 90.00

Refinement procedure

Resolution	72.550 - 2.800
R-factor	0.21858
Rwork	0.216
R-free	0.27555
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1O5Y
RMSD bond length	0.017
RMSD bond angle	1.635
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.550	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	8172
<I/σ(I)>	12	3.1
Completeness [%]	98.2	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.9		pH 6.90