1SJ3
Hepatitis Delta Virus Gemonic Ribozyme Precursor, with Mg2+ Bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2003-03-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9892 |
Spacegroup name | H 3 2 |
Unit cell lengths | 108.731, 108.731, 191.726 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.390 - 2.200 |
R-factor | 0.255 |
Rwork | 0.255 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 21056 |
Completeness [%] | 0.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | NaCl, MgCl2, MPD, Sodium Cacodylate, Spermine-HCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NaCl | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 1 | Sodium Cacodylate | ||
5 | 1 | 1 | Spermine-HCl | ||
6 | 1 | 2 | NaCl | ||
7 | 1 | 2 | MgCl2 | ||
8 | 1 | 2 | MPD |