1SIZ
Crystal structure of the [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.447, 51.637, 54.549 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.250 |
R-factor | 0.2413 |
Rwork | 0.239 |
R-free | 0.28880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vjw |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.100 | 0.220 |
Number of reflections | 6508 | |
<I/σ(I)> | 4.8 | |
Completeness [%] | 94.8 | 81.5 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 400, hexammine cobalt(III)-ions, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |