1SIR
The Crystal Structure and Mechanism of Human Glutaryl-CoA Dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 2000-07-08 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 116.722, 116.722, 128.034 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.820 - 2.600 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.24000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1siq |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.820 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 13764 | |
<I/σ(I)> | 11.3 | 2.2 |
Completeness [%] | 83.9 | 61 |
Redundancy | 2.4 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | MES buffer, PEG5KMME, Ammonium Sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |