1SIP

ALTERNATIVE NATIVE FLAP CONFORMATION REVEALED BY 2.3 ANGSTROMS RESOLUTION STRUCTURE OF SIV PROTEINASE

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	32.180, 62.520, 95.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.17
RMSD bond length	0.018
Refinement software	RESTRAIN

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.075 *
Total number of observations	25196 *
Number of reflections	4457 *
Completeness [%]	97.7 *	89.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	proteinase	3 (mg/ml)
2	1	reservoir	sodium acetate	100 (mM)
3	1	reservoir		0.2 (M)