1SIG
CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-25 |
| Detector | FUJI |
| Wavelength(s) | 0.9663, 0.9879 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 79.229, 79.229, 134.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.600 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.31500 |
| Structure solution method | MULTIWAVELENGTH ANOMALOUS DISPERSION |
| RMSD bond length | 0.014 |
| RMSD bond angle | 21.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.063 * | 0.270 * |
| Total number of observations | 76887 * | |
| Number of reflections | 13160 | |
| <I/σ(I)> | 15.1 | 2.5 |
| Completeness [%] | 94.9 | 78.3 |
| Redundancy | 5.84 | 4.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.1 | 15 * | Severinova, E., (1996) J.Mol.Biol., 263, 637. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12.5-15 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 25 (mM) | |
| 3 | 1 | drop | 0.25-0.35 (M) | ||
| 4 | 1 | drop | PEG8000 | 2.5-3.5 (%(w/v)) | |
| 5 | 1 | drop | DTT | 2.5 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 7 | 1 | reservoir | 0.5-0.7 (M) | ||
| 8 | 1 | reservoir | PEG8000 | 5-7 (%(w/v)) | |
| 9 | 1 | reservoir | DTT | 5 (mM) |
