1SI0
Crystal Structure of Mannheimia haemolytica Ferric iron-Binding Protein A in a closed conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.670, 192.941, 43.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.350 - 1.350 |
| R-factor | 0.15989 |
| Rwork | 0.159 |
| R-free | 0.17416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q35 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.096 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.350 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 85709 | |
| Completeness [%] | 95.0 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | HEPES, isopropanol, PEG 4000, glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
