1SI0
Crystal Structure of Mannheimia haemolytica Ferric iron-Binding Protein A in a closed conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.670, 192.941, 43.352 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 95.350 - 1.350 |
R-factor | 0.15989 |
Rwork | 0.159 |
R-free | 0.17416 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q35 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.096 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.350 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 85709 | |
Completeness [%] | 95.0 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | HEPES, isopropanol, PEG 4000, glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |