1SHX
Ephrin A5 ligand structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.210, 70.080, 58.460 |
Unit cell angles | 90.00, 95.94, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.100 |
Rwork | 0.224 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EprinB2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.360 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | |
Number of reflections | 16487 | |
<I/σ(I)> | 16.5 | |
Completeness [%] | 91.3 | 54.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 278 | PEG 3350, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |