1SHU
Crystal Structure of the von Willebrand factor A domain of human capillary morphogenesis protein 2: an anthrax toxin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9186 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.419, 57.419, 111.459 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.750 - 1.500 |
| R-factor | 0.1881 |
| Rwork | 0.187 |
| R-free | 0.21549 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sht |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.465 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.750 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.045 | 0.174 |
| Number of reflections | 30193 | |
| <I/σ(I)> | 33.3 | 7.7 |
| Completeness [%] | 98.2 | 90.5 |
| Redundancy | 7.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG3350, magnesium chloride, bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
