1SHS
SMALL HEAT SHOCK PROTEIN FROM METHANOCOCCUS JANNASCHII
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-11 |
Detector | BRANDEIS |
Spacegroup name | H 3 |
Unit cell lengths | 171.440, 171.440, 101.980 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.900 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.25100 |
Structure solution method | SIR + NCS AVERAGING |
RMSD bond length | 0.012 |
RMSD bond angle | 32.030 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.85) |
Refinement software | X-PLOR (3.85) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.037 | 0.431 |
Number of reflections | 24757 | |
<I/σ(I)> | 22.6 | 5.9 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.9 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.7 | Kim, K.K., (1998) J.Struct.Biol., 121, 76. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | sodium acetate | 0.2-0.3 (M) | |
3 | 1 | drop | MPD | 25-30 (%) | |
4 | 1 | drop | 0.02 (M) |