1SHQ
Crystal structure of shrimp alkaline phosphatase with magnesium in M3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2002-05-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.2825 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 171.007, 171.007, 84.098 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.530 - 2.000 |
R-factor | 0.21645 |
Rwork | 0.215 |
R-free | 0.23808 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.026 |
RMSD bond angle | 1.928 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.0) |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 42% (w/v) saturated ammonium sulfate, 100mM tris-maleic acid pH 5.6, 100 mM MgCl2, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |