1SHO
CRYSTAL STRUCTURE OF VANCOMYCIN AT ATOMIC RESOLUTION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 125 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-29 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 28.480, 28.480, 65.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.000 - 1.090 |
| R-factor | 0.1036 * |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX-96 |
| Refinement software | SHELXL-96 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.170 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Rmerge | 0.058 | 0.083 |
| Number of reflections | 11867 | |
| <I/σ(I)> | 27 | 19.7 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 13.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | PH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | HEPES | 5 (mM) | |
| 3 | 1 | drop | sodium acetate | 5 (mM) | |
| 4 | 1 | drop | sodium chloride | 1 (M) | |
| 5 | 1 | reservoir | sodium acetate | 10 (mM) | |
| 6 | 1 | reservoir | sodium chloride | 2 (M) |
