1SHN
Crystal structure of shrimp alkaline phosphatase with phosphate bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2002-02-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 170.676, 170.677, 83.925 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.640 - 2.150 |
R-factor | 0.18758 |
Rwork | 0.186 |
R-free | 0.21820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 1.969 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.150 |
Number of reflections | 66456 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 42% (w/v) saturated ammonium sulfate, 100mM tris-maleic acid pH 5.6, 1 mM MgCl2, 0.1mM ZnCl2, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |