1SHF

CRYSTAL STRUCTURE OF THE SH3 DOMAIN IN HUMAN FYN; COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF SH3 DOMAINS IN TYROSINE KINASES AND SPECTRIN

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	73.700, 48.600, 43.000
Unit cell angles	90.00, 98.10, 90.00

Refinement procedure

Resolution	5.000 * - 1.900
R-factor	0.18
Rwork	0.180
RMSD bond length	0.020
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.058 *
Total number of observations	37654 *
Number of reflections	10190 *
Completeness [%]	86.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop	DTT	1 (mM)
4	1	reservoir	sodium formate	4 (M)
5	1	reservoir	beta-mercaptoethanol	0.6 (%)