1SH7
Crystal structure of a cold adapted subtilisin-like serine proteinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-01-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0092 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.212, 36.881, 140.488 |
| Unit cell angles | 90.00, 97.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.840 |
| R-factor | 0.14632 |
| Rwork | 0.141 |
| R-free | 0.19659 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.521 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.870 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Number of reflections | 37883 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 100 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEC 4000, isopropanol, HEPES, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
