1SGW
Putative ABC transporter (ATP-binding protein) from Pyrococcus furiosus Pfu-867808-001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.066 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.982, 67.583, 72.768 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.22299 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.294 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.03) |
| Refinement software | REFMAC (refmac_5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.123 | |
| Number of reflections | 23651 | |
| Completeness [%] | 99.4 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | modified microbatch | 7.9 | 291 | 15% PEG-4000, 10% isopropanol, 10% glycerol, 100mM TRIS, pH 7.9, modified microbatch, temperature 291K |
