1SGR

LEU 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH STREPTOMYCES GRISEUS PROTEINASE B

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-01-10
Detector	XUONG-HAMLIN MULTIWIRE MARK II
Spacegroup name	P 1 21 1
Unit cell lengths	45.530, 54.660, 45.590
Unit cell angles	90.00, 119.19, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.139 *
RMSD bond length	0.022 *
RMSD bond angle	2.400 *
Data reduction software	BIOMOL
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.053
Total number of observations	41124 *
Number of reflections	17183 *
Completeness [%]	94.3 *	51.1 *
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG6000	2 (%)
3	1	drop	sodium potassium phosphate	50 (mM)
4	1	reservoir	PEG6000	2 (%)
5	1	reservoir	sodium potassium phosphate	50 (mM)