1SG2
Crystal structure of the periplasmic chaperone Skp
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93221 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.985, 83.911, 158.378 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.22608 |
| Rwork | 0.223 |
| R-free | 0.27914 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.647 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.076 | 0.427 |
| Number of reflections | 31346 | |
| <I/σ(I)> | 7 | 17.7 |
| Completeness [%] | 94.6 | 94.6 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 34.5% (v/v) ethanol, 7% (w/v) polyethylene glycol 1000, 50 mM Na-phosphate, 50 mM Na-citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
