1SFY
Crystal structure of recombinant Erythrina corallodandron Lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.240, 144.890, 127.660 |
| Unit cell angles | 90.00, 93.29, 90.00 |
Refinement procedure
| Resolution | 17.660 - 2.550 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fyu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.077 | 0.364 |
| Number of reflections | 49199 | |
| <I/σ(I)> | 18.1 | 18.1 |
| Completeness [%] | 95.7 | 96.2 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 25% Ammonium Sulphate 100mM MES, 0.025% Sodium Azide, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
