1SFT
ALANINE RACEMASE
Experimental procedure
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1994-11-26 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.590, 90.130, 85.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.26700 |
RMSD bond length | 0.019 |
RMSD bond angle | 26.050 * |
Data reduction software | RIGAKU |
Data scaling software | RIGAKU |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.046 |
Number of reflections | 52379 |
<I/σ(I)> | 9.3 |
Completeness [%] | 83.0 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8.5 | pH 8.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | PEG4000 | 30 (%(w/v)) | |
3 | 1 | drop | sodium acetate | 200 (mM) | |
4 | 1 | drop | Tris-HCl | 100 (mM) | |
5 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
6 | 1 | reservoir | sodium acetate | 200 (mM) | |
7 | 1 | reservoir | Tris-HCl | 100 (mM) |