1SFR

Crystal Structure of the Mycobacterium tuberculosis Antigen 85A Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	131.430, 288.662, 101.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 - 2.700
R-factor	0.222
Rwork	0.222
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dqz
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	52327
<I/σ(I)>	15
Completeness [%]	98.5	98
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Sodium Cacodylate, Sodium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K