1SFE
ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Detector technology | DIFFRACTOMETER |
| Detector | WEISSENBERG |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.200, 45.950, 46.400 |
| Unit cell angles | 90.00, 114.06, 90.00 |
Refinement procedure
| Resolution | 39.000 - 2.100 |
| R-factor | 0.219 * |
| Rwork | 0.219 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 * |
| High resolution limit [Å] | 2.100 * |
| Rmerge | 0.058 |
| Total number of observations | 19437 * |
| Number of reflections | 9017 |
| Completeness [%] | 86.0 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | |||
| 1 | Microdialysis * | |||
| 1 | Microdialysis * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5-50 (mg/ml) | |
| 2 | 1 | 1 | sodium acetate | 200-500 (mM) | |
| 3 | 1 | 1 | dithiothreitol | 10 (mM) | |
| 4 | 1 | 1 | Tris-acetate | 10-20 (mM) | |
| 5 | 1 | 2 | dithiothreitol | 10 (mM) | |
| 6 | 1 | 2 | glucose | 300 (mM) | can be replaced by maltose |
| 7 | 1 | 2 | Tris-acetate | 10 (mM) |
