1SF9
Crystal Structure of Bacillus subtilis YfhH Protein : Putative Transcriptional Regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.142, 46.486, 50.511 |
| Unit cell angles | 90.00, 114.98, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.710 |
| Rwork | 0.172 |
| R-free | 0.20380 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.770 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.044 | 0.092 |
| Number of reflections | 12888 | |
| <I/σ(I)> | 39 | 21.6 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 1.8M Hexanediol, 0.2M Calcium Chloride, 0.5M Sodium Chloride, 0.1M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
