1SF8
Crystal structure of the carboxy-terminal domain of htpG, the E. coli Hsp90
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1271, 0.9791, 0.9790 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 103.516, 103.516, 249.738 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.900 - 2.600 |
R-factor | 0.225 |
Rwork | 0.223 |
R-free | 0.26100 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.630 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 79708 | |
<I/σ(I)> | 1.5 | |
Completeness [%] | 96.3 | 70.5 |
Redundancy | 5.1 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium malonate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |