1SF5
Structure of oxidized state of the P94A mutant of amicyanin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2002-08-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.396, 55.434, 27.095 |
Unit cell angles | 90.00, 95.17, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.100 |
R-factor | 0.127 |
Rwork | 0.127 |
R-free | 0.16600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | PDB file 1AAC |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.120 |
High resolution limit [Å] | 1.100 | 1.100 |
Number of reflections | 32824 | |
<I/σ(I)> | 24.5 | 2.8 |
Completeness [%] | 96.7 | 71.5 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5 | 293 | 3.6 M monobasic sodium phosphate, 0.4 M dibasic potassium phosphate, pH 5.0, EVAPORATION, temperature 293.0K |