1SEV

Mature and translocatable forms of glyoxysomal malate dehydrogenase have different activities and stabilities but similar crystal structures

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1998-08-28
Detector	CUSTOM-MADE
Spacegroup name	P 41 21 2
Unit cell lengths	96.800, 96.800, 213.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.550
R-factor	0.21
Rwork	0.207
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mdh
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	PEG 800, Sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K