1SEM
STRUCTURAL DETERMINANTS OF PEPTIDE-BINDING ORIENTATION AND OF SEQUENCE SPECIFICITY IN SH3 DOMAINS
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-07-24 |
| Detector | MACSCIENCE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.912, 68.410, 35.029 |
| Unit cell angles | 90.00, 94.71, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.540 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.080 |
| Number of reflections | 6912 |
| Completeness [%] | 92.5 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 15 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 52.5 (%sat) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) |
