1SEF
Crystal structure of cupin domain protein EF2996 from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.01 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 104.984, 104.984, 159.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.050 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.470 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.052 | 0.228 |
| Number of reflections | 27665 | |
| <I/σ(I)> | 39.6 | 9.2 |
| Completeness [%] | 97.6 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 278 | MPD, calcium chloride, bis-Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K |
