1SED
Crystal Structure of Protein of Unknown Function YhaL from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-09 |
| Detector | SBC-2 |
| Wavelength(s) | 1.282, 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.784, 64.550, 64.503 |
| Unit cell angles | 111.37, 107.19, 107.13 |
Refinement procedure
| Resolution | 29.810 - 2.100 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.20900 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | autoSHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.810 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.061 | 0.345 |
| Number of reflections | 41848 | |
| <I/σ(I)> | 8.3 | 3.2 |
| Completeness [%] | 98.3 | 97.4 |
| Redundancy | 4.24 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | sodium fluoride, PEG 3350, 2-methyl 2,4 pentanediol, sodium cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
