1SE6
Crystal Structure of Streptomyces Coelicolor A3(2) CYP158A2 from antibiotic biosynthetic pathways
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2003-03-18 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.554, 79.206, 87.430 |
| Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
| Resolution | 35.900 - 1.750 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s1f |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.600 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.900 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.058 | 0.180 |
| Number of reflections | 73147 | |
| <I/σ(I)> | 21.8 | 6.5 |
| Completeness [%] | 89.2 | 92.4 |
| Redundancy | 18.6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 2000 monomethylether, tetrahydrocloride spermine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
