1SDZ
Crystal structure of DIAP1 BIR1 bound to a Reaper peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-01 |
| Detector | FUJI |
| Spacegroup name | H 3 |
| Unit cell lengths | 84.800, 84.800, 49.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.780 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.351 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.850 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.056 | |
| Number of reflections | 12620 | |
| Completeness [%] | 99.0 | 92.1 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | CHES, sodium citrate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
