1SDJ
X-RAY STRUCTURE OF YDDE_ECOLI NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ET25.
Replaces: 1P9VExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97929, 0.97970, 0.97702 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 125.047, 130.659, 48.555 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.300 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.26000 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 24.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.075 | 0.247 |
Number of reflections | 32871 | |
Completeness [%] | 89.5 * | 85.6 |
Redundancy | 4.3 | 27 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | ligand | 10 (mM) | |
3 | 1 | reservoir | PEG4000 | 24-30 (%(w/v)) | |
4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH4.4-5.0 |
5 | 1 | reservoir | 0.2 (M) |