1SDJ
X-RAY STRUCTURE OF YDDE_ECOLI NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ET25.
Replaces: 1P9VExperimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97929, 0.97970, 0.97702 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 125.047, 130.659, 48.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.300 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.247 |
| Number of reflections | 32871 | |
| Completeness [%] | 89.5 * | 85.6 |
| Redundancy | 4.3 | 27 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ligand | 10 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 24-30 (%(w/v)) | |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) | pH4.4-5.0 |
| 5 | 1 | reservoir | 0.2 (M) |
