1SDI
1.65 A structure of Escherichia coli ycfC gene product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-14 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.527, 62.649, 68.816 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.650 |
| R-factor | 0.1505 |
| Rwork | 0.150 |
| R-free | 0.18386 |
| Structure solution method | isomorphous replacement |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.105 | 0.477 |
| Number of reflections | 21058 | |
| <I/σ(I)> | 17.6 | 2.1 |
| Completeness [%] | 92.7 | 60.8 |
| Redundancy | 6.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 273 | PEG 3350, PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
