1SDD

Crystal Structure of Bovine Factor Vai

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.935
Spacegroup name	P 21 21 21
Unit cell lengths	63.370, 86.560, 229.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.262
Rwork	0.252
R-free	0.29600
Structure solution method	MIR + MAD
RMSD bond length	0.019
RMSD bond angle	1.940
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.049	0.206
Number of reflections	28745
<I/σ(I)>	7.2	2.3
Completeness [%]	88.8	80.8
Redundancy	4.2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	285	PEG3350, MAGNESIUM CHLORIDE, CALCIUM CHLORIDE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 285K
1	VAPOR DIFFUSION, SITTING DROP	7	285	PEG3350, MAGNESIUM CHLORIDE, CALCIUM CHLORIDE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 285K
1	VAPOR DIFFUSION, SITTING DROP	7	285	PEG3350, MAGNESIUM CHLORIDE, CALCIUM CHLORIDE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 285K
1	VAPOR DIFFUSION, SITTING DROP	7	285	PEG3350, MAGNESIUM CHLORIDE, CALCIUM CHLORIDE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 285K
1	VAPOR DIFFUSION, SITTING DROP	7	285	PEG3350, MAGNESIUM CHLORIDE, CALCIUM CHLORIDE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 285K