1SCM
STRUCTURE OF THE REGULATORY DOMAIN OF SCALLOP MYOSIN AT 2.8 ANGSTROMS RESOLUTION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS |
| Synchrotron site | NSLS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.500, 87.000, 55.500 |
| Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.760 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.113 * |
| Total number of observations | 30711 * |
| Number of reflections | 10352 * |
| Completeness [%] | 91.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | drop | 0.1 (mM) | ||
| 4 | 1 | drop | HEPES | 75 (mM) | |
| 5 | 1 | drop | PEG4000 | 7-8 (%) | |
| 6 | 1 | reservoir | PEG4000 | 14-16 (%(w/v)) |
