1SCH

PEANUT PEROXIDASE

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1991-09
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	48.100, 97.200, 146.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.560
R-factor	0.199
Rwork	0.199
R-free	0.26200
RMSD bond length	0.006
RMSD bond angle	20.619 *
Data reduction software	XENGEN ((HOWARD)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.560	2.560 *
Rmerge	0.086
Total number of observations	56127 *
Number of reflections	19674
Completeness [%]	86.0 *	41 *
Redundancy	2.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7		pH 6.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	12 (%)
2	1	drop	enzyme solution	15 (mg/ml)
3	1	drop	sodium phosphate	0.35 (M)
4	1	drop	PEG4000	12 (%)
5	1	drop	n-octyl-beta-D-glucoside	35 (%)