1SC0
X-ray Structure of YB61_HAEIN Northeast Structural Genomics Consortium Target IR63
Replaces: 1O0IExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.500, 60.900, 70.960 |
| Unit cell angles | 90.00, 134.50, 90.00 |
Refinement procedure
| Resolution | 19.520 - 1.700 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.102 | 0.261 |
| Number of reflections | 65061 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 95.5 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 200 mM potassium sodium tartrate tetrahydrate, 18% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
