1SBM
Crystal Structure of Reduced Mesopone cytochrome c peroxidase (R-isomer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 116 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.826, 75.666, 50.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 1.720 - 1.690 |
| Rwork | 0.181 |
| R-free | 0.20040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s73 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.272 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.062 | 0.597 |
| Number of reflections | 47110 | |
| <I/σ(I)> | 20.98 | 1.8 |
| Completeness [%] | 99.0 | 96.9 |
| Redundancy | 16.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 2-methyl-2,4-pentanediol (MPD), 100mM potassium phosphate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
