1SBI
SUBTILISIN BPN' 8397 (E.C. 3.4.21.14) MUTANT (M50F, N76D, G169A, Q206C, N218S)
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-03-29 |
Detector | RIGAKU |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.510, 79.340, 37.240 |
Unit cell angles | 90.00, 114.32, 90.00 |
Refinement procedure
Resolution | 17.000 - 2.200 |
R-factor | 0.164 |
Rwork | 0.164 |
R-free | 0.16300 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.785 |
Data reduction software | TEXSAN |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 17.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 11759 |
Completeness [%] | 100.0 |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | sodium cacodylate | 5 (mM) | |
3 | 1 | drop | PEG3400 | 15 (%(w/v)) | |
4 | 1 | drop | ammonium acetate | 100 (mM) | |
5 | 1 | reservoir | PEG3400 | 30 (%(w/v)) | |
6 | 1 | reservoir | ammonium acetate | 200 (mM) | |
7 | 1 | reservoir | sodium acetate | 100 (mM) |