1SBH
SUBTILISIN BPN' 8397+1 (E.C. 3.4.21.14) MUTANT (M50F, N76D, G169A, Q206C, N218S, K256Y)
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-07-10 |
Detector | RIGAKU |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.560, 79.530, 37.250 |
Unit cell angles | 90.00, 114.48, 90.00 |
Refinement procedure
Resolution | 17.000 - 1.800 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.19200 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.694 |
Data reduction software | TEXSAN |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 17.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.113 |
Number of reflections | 21228 |
Completeness [%] | 100.0 |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.5-5 (mg/ml) | |
2 | 1 | drop | sodium cacodylate | 5 (mM) | |
3 | 1 | drop | PEG3400 | 8.5 (%(w/v)) | |
4 | 1 | drop | 2-propanol | 8.5 (%(v/v)) | |
5 | 1 | drop | sodium citrate | 50 (mM) | |
6 | 1 | reservoir | PEG3400 | 17 (%(w/v)) | |
7 | 1 | reservoir | 2-propanol | 17 (%(v/v)) | |
8 | 1 | reservoir | sodium citrate | 100 (mM) |