1SBE
SOYBEAN AGGLUTININ FROM GLYCINE MAX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-12 |
| Detector | SIEMENS |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 143.300, 143.300, 107.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 99.000 - 2.800 |
| Rwork | 0.212 |
| Structure solution method | MOLECULAR DYNAMICS VS 1SBA |
| RMSD bond length | 0.013 |
| RMSD bond angle | 19.920 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.139 | 0.540 |
| Total number of observations | 61134 * | |
| Number of reflections | 16581 | |
| <I/σ(I)> | 4.1 | 0.6 |
| Completeness [%] | 84.0 | 67 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | 4 * | Dessen, A., (1995) Biochemistry, 34, 4933. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | SBA | 17 (mg/ml) | |
| 10 | 1 | reservoir | 0.5 (M) | ||
| 2 | 1 | drop | sugar | 0.755 (mM) | |
| 3 | 1 | drop | HEPES | 0.1 (M) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | drop | 0.375 (M) | ||
| 7 | 1 | reservoir | HEPES | 0.1 (M) | |
| 8 | 1 | reservoir | 1 (mM) | ||
| 9 | 1 | reservoir | 1 (mM) |
