1SBD
SOYBEAN AGGLUTININ COMPLEXED WITH 2,4-PENTASACCHARIDE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 287 |
Detector technology | AREA DETECTOR |
Collection date | 1994-10-09 |
Detector | SIEMENS |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 144.600, 144.600, 107.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.520 |
Rwork | 0.205 |
Structure solution method | RIGID BODY REFINEMENT VS 1SBA |
RMSD bond length | 0.017 |
RMSD bond angle | 18.573 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | X-PLOR |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.670 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmerge | 0.140 | 0.411 |
Total number of observations | 112590 * | |
Number of reflections | 18788 | |
<I/σ(I)> | 7.5 | 2.7 |
Completeness [%] | 82.0 | 49 * |
Redundancy | 5.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 | 4 * | Dessen, A., (1995) Biochemistry, 34, 4933. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | SBA | 17 (mg/ml) | |
10 | 1 | reservoir | 0.5 (M) | ||
2 | 1 | drop | sugar | 0.755 (mM) | |
3 | 1 | drop | HEPES | 0.1 (M) | |
4 | 1 | drop | 1 (mM) | ||
5 | 1 | drop | 1 (mM) | ||
6 | 1 | drop | 0.375 (M) | ||
7 | 1 | reservoir | HEPES | 0.1 (M) | |
8 | 1 | reservoir | 1 (mM) | ||
9 | 1 | reservoir | 1 (mM) |