1SBB
T-CELL RECEPTOR BETA CHAIN COMPLEXED WITH SUPERANTIGEN SEB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-08 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.200, 83.650, 82.980 |
| Unit cell angles | 90.00, 93.04, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.400 |
| R-factor | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SE4 AND 1BEC |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.092 | 0.360 |
| Total number of observations | 325939 * | |
| Number of reflections | 34934 | |
| <I/σ(I)> | 17 | 4 |
| Completeness [%] | 91.5 | 79.1 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 9-12 (%(w/v)) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) |
