1SAY

L-ALANINE DEHYDROGENASE COMPLEXED WITH PYRUVATE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-01-21
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	122.400, 122.400, 183.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.18 *
Rwork	0.180
R-free	0.25000
Structure solution method	ISOMORPHOUS WITH NATIVE ENZYME
RMSD bond length	0.015
RMSD bond angle	18.100 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	CCP4
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.031	0.200
Number of reflections	63279
<I/σ(I)>	16.7	3.5
Completeness [%]	96.2	99.1
Redundancy	2.1	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7		pH 6.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	drop	MES	0.1 (M)
3	1	reservoir	PEG20000	6-10 (%)
4	1	reservoir	MES	0.1 (M)