1SAV
HUMAN ANNEXIN V WITH PROLINE SUBSTITUTION BY THIOPROLINE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 280 |
Detector technology | IMAGE PLATE |
Collection date | 1997-02 |
Detector | MARRESEARCH |
Spacegroup name | H 3 |
Unit cell lengths | 99.600, 99.600, 97.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1avr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.411 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.550 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.049 | |
Number of reflections | 12532 | |
<I/σ(I)> | 9.5 | |
Completeness [%] | 99.3 | 95.8 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 * | PROTEIN WAS CRYSTALLIZED FROM 2.1 M AMMONIUM SULFATE, 0.1 M TRIS-HCL, PH 8.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | drop | Mops | 3 (mM) | |
3 | 1 | drop | 20 (mM) | ||
4 | 1 | drop | precipitant | 0.0015ml | |
5 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
6 | 1 | reservoir | Tris-chloride | 0.1 (M) |